Figure 1.

Amplicon size and locations of informative CpG sites in amplicons of the Nell-1 gene. (a) The sequences of targeted gene. The amplified region was colored in gray. Transcription start sequences were labeled in frame and transcription sites were labeled in yellow. CpG sites in amplicons appear in red and single line represented separate detection. Double line showed testing with the adjacent site. Point line showed adjacent three-site testing. No line showed that CpG sites cannot be detected in amplified sequences. (b) The amplicon map and the locations of CpG sites.