| Step | Total Time(min) | Flow Rate(μl/min) | A (%) | B (%) |
| 0 | 0.00 | 300 | 97.0 | 3.0 |
| 1 | 0.50 | 300 | 97.0 | 3.0 |
| 2 | 0.60 | 300 | 5.0 | 95.0 |
| 3 | 1.20 | 300 | 5.0 | 95.0 |
| 4 | 2.00 | 300 | 97.0 | 3.0 |
| 5 | 5.50 | 300 | 97.0 | 3.0 |
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