Fig 5. RNA blot analysis of maize CYP97C19 transgene expression in A. thaliana wild-type, lut1 mutant and three different transgenic lines transformed with ZmCYP97C19 driven by the CaMV 35S promoter.

Each lane was loaded with 20 μg total RNA from rosette leaves. Ribosomal RNA stained with ethidium bromide is shown as a loading control. The blot was hybridized with a ZmCYP97C19 probe. WT, wild type (Col-0); Lut1, lut1 mutant; L1, line 1; L2, line 2; L3, line 3.