Figure 8.

Evaluations of ROS produced by PMA-activated macrophages with the CM-H2DCF-DA probe. MDM-LPS response of controls to the NADPH activator PMA is shown in (a). The kinetic ROS production of fullerene 1 (b) and fullerene 2 (c) pretreated cells (gray symbols) is compared to that of untreated control (black symbols), and all (untreated and treated) were stimulated with 0.1 μM PMA. Each point represents the mean value ± SEM of at least three independent determinations, obtained by the ratio of the fluorescence emitted by PMA stimulated cells/basal fluorescence, measured as MFI at each time of x-axes. ^∗ P < 0.05 versus untreated controls, post-ANOVA Student-Newman-Keuls multiple comparison test.