Table 3.
Flow cytometric analysis of the fullerene derivatives interaction with monocytes and macrophages.
| Monocytes | MDM-LPS | |||
|---|---|---|---|---|
| % | MFI | % | MFI | |
| 0 μM | 0 ± 0.01 | 0 ± 0.01 | 0.16 ± 0.04 | 3.1 ± 0.4 |
|
| ||||
| 5 μM fullerene 1-FITC | 3.8 ± 0.3 | 3.0 ± 0.05 | 94.1 ± 0.06 | 34.7 ± 6.3∗∗∗ |
| 10 μM fullerene 1-FITC | 12.6 ± 1.2 | 3.2 ± 0.01 | 98.6 ± 0.19 | 76.4 ± 2.8∗∗∗,§ |
|
| ||||
| 5 μM fullerene 2-FITC | 0.3 ± 0.03 | 3.1 ± 0.04 | 93.50 ± 0.23 | 18.7 ± 0.8∗∗ |
| 10 μM fullerene 2-FITC | 1.8 ± 0.19 | 3.4 ± 0.08 | 96.93 ± 0.36 | 31.5 ± 1.0∗∗∗,§ |
Cells (1 × 106/mL) were exposed for 24 h to 5–10 μM of fullerene derivatives: FITC tagged and subsequently recovered and run by flow cytometer. The percentage of positive cells and the mean fluorescent intensity of FL1 channel are reported. Each value is the mean ± SEM of triplicate samples. ∗∗ P < 0.01, ∗∗∗ P < 0.001 versus untreated controls; § P < 0.05 versus the lower concentration (5 μM). Student-Newman-Keuls multiple comparisons test, ANOVA.