Table 3. Real-time PCR confirmation of the relative expression of genes showing differential expression between the two gonad types.
| Unigene ID | Illumina (O/T)a | qRT-PCR (O/T)b | Nr Annotation |
|---|---|---|---|
| 75530 | 14.44 | 29.00 | zona pellucida glycoprotein 3 |
| 59852 | 13.45 | 16.50 | zona pellucida glycoprotein AX |
| 57743 | 7.22 | 7.69 | spermidine synthase |
| 54618 | 9.58 | 20.00 | hatching enzyme |
| 73936 | 7.61 | 12.50 | Pou2 |
| 75228 | 5.59 | 11.11 | bucky ball |
| 71078 | 5.11 | 20.00 | E-type cyclin |
| 60411 | 4.42 | 16.67 | SRY (sex determining region Y)-box 3 |
| 74439 | 3.90 | 12.50 | growth/differentiation factor 9 |
| 74160 | -7.47 | -10.90 | predicted: vitrin-like |
a The relative-fold expression determined by Illumina transcriptome analysis.
b The relative-fold expression validated by qRT-PCR.
O/T: The relative transcription level of genes in ovary compared to that in testis. O, Ovary; T, Testis.