Fig 2. RhoA is not essential for CCSP-promoter driven, K-Ras^G12D-induced, lung adenoma formation in mice.

Rho^WT and RhoA^flox/flox mice were mated with CCSP-Cre mice on a K-Ras^G12D background. (A & B) K-Ras^G12D lungs by H&E (bars represent 1mm and 200μm for panels A & B respectively). (C) Immunohistochemistry for pERK^{Thr202/Tyr204} (brown) with hematoxylin counterstain (bars represent 100μm). (D) Immunohistochemistry for RhoA (brown) with hematoxylin counterstain (bars represent 100μm). (E) Quantification of the RhoA-status of adenomas as assessed by immunohistochemistry. Greater than 30 tumors were counted from four mice per group.