Fig 4. Neither RhoA nor RhoC is required for K-Ras^G12D-induced adenoma formation in a CCSP-Cre model.

Rho^WT, RhoC^-/- and DKO mice were mated with CCSP-Cre mice on a K-Ras^G12D background. (A & B) K-Ras^G12D lungs by H&E (bars represent 1mm and 200μm for panels A & B respectively). (C) Immunohistochemistry for pERK^{Thr202/Tyr204} (brown) with hematoxylin counterstain (bars represent 100μm). (D) Immunohistochemistry for RhoA (brown) with hematoxylin counterstain (bars represent 100μm). (E) Quantification of the RhoA-status of adenomas as assessed by immunohistochemistry. Greater than 30 tumors were counted from four mice per group.