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. 2015 Jun 1;10(6):e0128940. doi: 10.1371/journal.pone.0128940

Fig 4. Microneutralization titers of vaccinated mouse sera against 100TCID50 of RG-H7N7 and RG-H7N9 viruses.

Fig 4

Groups of mice (n = 10) were subcutaneously immunized with a constant dosage of 100 μl containing 2.5 μg of live Bac-HA, or Bac-HAm constructs T143A or T198A or I211V or T143A-T198A or T198A-I211V or I211V-T143A or wild- type baculovirus (PFU 1X108), controls on day 0 and 28. Serum samples were collected on day 42 from each experimental mice group (Five mice/Group) for measuring the serum neutralization antibody titers against H7N7 (A/Netherlands/219/03) or H7N9 (A/Shanghai/2/2013) viruses. Neutralizing titers are arithmetic mean of the highest dilution of serum which yielded a 50% reduction in virus infectivity. a-when compared with wild-type H7N7 Bac-HA vaccine group. Each point represents the arithmetic mean value (n = 5) ± SD (**P<0.001).