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. 2015 May 19;2015:159132. doi: 10.1155/2015/159132

Figure 2.

Figure 2

YSC-ZDC inhibited the proliferation of Hep3B cells. (a) YSC-ZDC inhibited cell proliferation in Hep3B cells. Cells were treated with YSC-ZDC (0–500 μg/mL) or 5-Fu (0–40 μg/mL) for the indicated times. Cell proliferation was determined by MTT assay. Data are shown as means ± S.E.M of three independent experiments. (b) Cells were treated with 100 μg/mL YSC-ZDC or 20 μg/mL 5-Fu for 48 h and then fixed with 2.5% glutaraldehyde and postfixed in 1% osmium tetroxide. Ultrathin sections were examined using transmission electron microscopy. (c) Cells were treated with 100 μg/mL YSC-ZDC or 20 μg/mL 5-Fu for 48 h and incubated with Hoechst 33258 (5 μg/mL) for 10 min. Morphological changes of nuclear were visualized and analyzed using an inverted fluorescence microscope.