Table 1. Enzyme activities of digestive enzyme supplements and purified enzymes.
| Enzyme assay | ||||
|---|---|---|---|---|
| Digestive enzyme | Capsule content (mg) | HUT/mg (pH 4.7) | LAPU/g (pH 7.0) | DAP/g (pH 7.5) |
| Supplement A | 695 | 47 | 67 | 1.09 |
| Supplement B | 335 | 210 | 273 | 0.60 |
| Supplement C | 465 | 210 | 293 | 0.55 |
| Supplement D | 395 | 290 | 335 | 0.78 |
| Supplement E | 425 | 81 | 76 | 0.27 |
| AN-PEP (DSM) | 275 # | 0.34 | 0 | 0.25* |
| DPPIV (DSM) | n.d. | n.d. | 165 | |
| LAP (AB Enzymes) | 0.063 | 378 | 0 | |
The content of one capsule of supplement was weighed and the enzyme activities measured as specified in Materials and Methods. In the case of HUT, activity per capsule ((capsule content in mg) * (specific activity in HUT/mg)) matches with the product specification sheet within a factor three (see S1 Text)
# AN-PEP is at present not commercially available in the form of a capsule; 275 mg is the intended capsule content
* AN-PEP was also tested in a modified DAP assay at pH 4.6 (instead of 7.5) and using N-carbobenzoxy-Gly-Pro-p-nitroanilide as a substrate. The specific activity of AN-PEP in this assay was 45 units/g.
n.d., not determined.