. Author manuscript; available in PMC: 2015 Jun 3.

Published in final edited form as: Alcohol Clin Exp Res. 2013 Jul 29;37(12):2002–2010. doi: 10.1111/acer.12192

Table 3.

Glycine EC50, Hill Slopes, and Maximal Currents for α3P185L-GlyRs

Subunit	EC50	Hill slope	I_max (nA)
α3 (n = 6)	837.1 ± 40	1.57 ± 0.27	7,715 ± 460
α3P185L (n = 7)	59.03 ± 7^***	0.59 ± 0.47^**	6,814 ± 750

Glycine concentration–response curves were generated for α3-WT and mutant P185L GlyRs. Introduction of the P185L mutation, which corresponds to RNA editing that occurs in vivo, produced a leftward shift in the concentration–response curve significantly reducing the glycine EC50, t(8) = 14.29, p < 0.0001, and the Hill slope, t(11) = 3.86, p = 0.003. However, the mutation had no significant effect on the maximal glycine-activated currents, t(11) = 0.98, p = 0.4.

^***

p < 0.0001,

^**

p < 0.01.