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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Clin Cancer Res. 2015 Mar 4;21(11):2580–2590. doi: 10.1158/1078-0432.CCR-14-2191

Figure 2. YAP UP-Regulates EGFR Expression in both Normal and Malignant EC Cells.

Figure 2

A. SKGT-4, YES-6 and KATO-TN cells were transduced with lentiviral plasmid containing inducible YAP1 cDNA (PIN20YAP1). YAP expression was induced by doxycycline to the culture medium at 1μg/ml. Immunoblotting using antibodies against YAP, EGFR and IGFR were performed (left). Immunoblotting of YAP, EGFR was performed in JHESO cells with two independent YAP shRNAs (YAP sh2 and YAPsh3) clones (right). B. Immunoblotting of YAP, EGFR was performed in SKGT-4 (PIN20YAP) cells with (DOX+) or without (DOX-) YAP induction and knockdown YAP in SKGT-4 (PIN20YAP) DOX+ induced cells. C. Immunofluorescent staining of YAP and EGFR in SKGT-4 cells that were transduced with inducible YAP (PIN20YAP) with or without doxycycline induction at 1μg/ml. D. YAP and EGFR was detected by immunoblotting in 293T cells transfected with either mutant YAPS127A or wt YAP expression vectors (left). YAP and EGFR was detected by immunoblotting in primary murine esophageal cells transduced with lentiviral plasmid containing inducible YAP1 cDNA (PIN20YAP1) (middle). EGFR expression was examined by immunoblotting in B299 cells and tumor cell lines isolated from Mst1/2-/- mouse tumor tissues (right).