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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Clin Cancer Res. 2015 Mar 4;21(11):2580–2590. doi: 10.1158/1078-0432.CCR-14-2191

Figure 3. YAP increases EGFR transcription through an intact Tead binding site.

Figure 3

A. mRNA levels of YAP and EGFR were determined by Q-PCR in SKGT-4 cells transduced with lentiviral plasmid containing inducible YAP1 cDNA (PIN20YAP1) and induce YAP with or without doxycycline at 1 μg/ml. B. Transient transfection of EGFR luciferase promoter reporter into SKGT-4 (PIN20YAP) cells with or without induced YAP by doxycycline at 1 μg/ml;. EGFR luciferase reporter activity was measured after 48 hours (left panel). Co-transfection of EGFR luciferase promoter reporter with YAP or YAP plus TEAD into 293T cells; EGFR luciferase reporter activity was detected after 48 hours (right panel). C. Wide type Tead binding site and a mutation (ATT-TCG) or deletion of the TEAD binding sites were depicted (left panel). Co-transfection of EGFR luciferase promoters (wide type or mutated or deleted in the Tead binding site) with YAP or control vector into 293T cells; EGFR luciferase reporter activity was detected after 48 hours (right panel). D. Co-transfection of EGFR luciferase promoters (wide type or mutated or deleted in the Tead binding site) with YAP or control vector into SKGT-4(PIN20YAP) with (DOX+) or without (DOX-) YAP induction; EGFR luciferase reporter activity was detected after 48 hours. For all experiments, values shown represent the mean and SD of at least triplicate assays (**p<0.01).