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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Alcohol Clin Exp Res. 2015 Apr 23;39(6):969–979. doi: 10.1111/acer.12727

Fig. 1.

Fig. 1

Effects of chronic ethanol feeding on hepatic expression of Wnt pathway-related genes. Adult male Long Evans rats were fed with isocaloric liquid diets containing 0% (C) or 37% ethanol (E) by caloric content for 8 weeks. A Quantigene 2.0 Multiplex Assay was used to quantify mRNA levels of (A) Wnt1 and Wnt3 ligands and Porcn, (B) Fzd3 receptor, (C) Lef1, Tcf7l2 Wnt transcription factors and Bcl9 co-factor, (D) Axin2, Ccnd1, Jun, and Wisp1 Wnt target genes, (E) Wif1, Sfrp2 and Sfrp5 Wnt ligand binding proteins, and (F) Apc2, Fgf4 andSenp2. Gene expression level is reflected by mean fluorescent intensity (MFI; arbitrary units). Graphs depict the net MFI ± S.D. with results normalized to housekeeping genes. *p < 0.05 vs. control-fed rats (n=3-5).