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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Alcohol Clin Exp Res. 2015 Apr 23;39(6):969–979. doi: 10.1111/acer.12727

Fig. 3.

Fig. 3

Effects of chronic ethanol feeding on Wnt pathway transcription regulators and responsive target genes expression during liver regeneration following PH. Livers harvested at different time point post-PH from control (C) or ethanol (E) fed rats were obtained to measure time-course dependent shifts in (A) Lef1, Tcf7l2 and Bcl9, (B) Axin2, Ccnd1 and Wisp1 mRNA expression using the Quantigene 2.0 Multiplex Assay. Gene expression level is reflected by mean fluorescent intensity (MFI; arbitrary units). Graphs depict time-dependent shifts in net MFI ± S.D. with results normalized to housekeeping genes. Inter-group comparisons were made using two-way repeated measures ANOVA with Bonferroni's pos-hoc test, *p < 0.05 vs. control-fed rats (n = 3-5).