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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Alcohol Clin Exp Res. 2015 Apr 23;39(6):969–979. doi: 10.1111/acer.12727

Fig. 4.

Fig. 4

Effects of chronic ethanol feeding on Wnt ligand binding proteins and other components expression following PH. Adult male Long Evans rats were fed with isocaloric liquid diets containing 0% (C) or 37% ethanol (E) for 8 weeks, after which they underwent 2/3 PH. Livers harvested 0, 18, 24, 30, 48, 72, or 168 hours post-PH were obtained to measure time-course dependent shifts in (A) Sfrp2, Sfrp5 and Wif1, (B) Apc2, Senp2 and Fgf4 mRNA expression using the Quantigene 2.0 Multiplex Assay. Gene expression level is reflected by mean fluorescent intensity (MFI; arbitrary units). Graphs depict time-dependent shifts in net MFI ± S.D. with results normalized to housekeeping genes. Inter-group comparisons were made using two-way repeated measures ANOVA with Bonferroni's pos-hoc test, *p < 0.05 vs. control-fed rats (n = 3-5).