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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Cancer Res. 2015 Apr 8;75(11):2363–2374. doi: 10.1158/0008-5472.CAN-14-2928

Figure 1. Plk1 down-regulates SUZ12 and ZNF198 proteins.

Figure 1

A. (left panel) 4pX-1 cells, transiently transfected with SUZ12-HA and ZNF198-GFP encoding plasmids, were grown with (+) or without (−) HBx expression by tetracycline removal for 48h; whole cell extracts (WCE) were immunoblotted with GFP or HA antibodies. (Right panel) WCE from 4pX-1 cells treated with nocodazole (100 nM) for 12h and grown with (+) or without (−) HBx expression for 16h, were immunoblotted with ZNF198 or SUZ12 antibodies; B. HEK293T cells transiently transfected with SUZ12-HA and ZNF198-GFP encoding plasmids, were grown with (+) or without (−) BI2536 (250 nM) for 12h or MG132 (2.5 μM). (Left panel) DMSO serves as vehicle control. (Right panel) Transfected cells were synchronized in G2/M by addition of nocodazole (100 nM) for 12 hr. Lysates were immunoblotted with HA or GFP antibodies C. Immunoblots of indicated proteins using WCE from HepAD38 cells grown with (+) or without (−) HBV replication by tetracycline removal for 5 and 10 days. D. Immunofluorescence microscopy of indicated proteins, +/- HBV replication in HepAD38 cells.