Skip to main content
. 2015 Jun 1;10(6):e0127551. doi: 10.1371/journal.pone.0127551

Fig 3. Intracellular ROS levels assayed with DCFH-DA fluorescent probe in porcine granulosa cells treated with ZEA.

Fig 3

A (A’), Control (untreated); B (B’), 15 μM ZEA; C (C’), 30 μM ZEA; D (D’), 60 μM ZEA. Intracellular ROS was measured by flow cytometry (A-D) and fluorescent imaging (A’-D’) using DCFH-DA probe. For better clarity, a guidance line (red) is drawn through histograms for comparison. E, Average intensity from DCFH-DA. The results are presented as mean ± SD. Asterisk (*) indicates significant difference (P<0.05).