Figure 5.

NGF-evoked increases in TRPV1-active oxidized lipids in spinal cord were attenuated by spinal injection of NDGA and BEL. Lumbar spinal cord were rapidly removed from untreated rats, NGF-treated rats, and NGF-treated rats cotreated with NDGA (5 μg, i.t.) or BEL (30 μg, i.t.) on day 8 (n = 7–10/group). Lipids were extracted in methanol-containing isotope-labeled standards for MS analysis of LA and AA (A), HODE and oxoODE metabolites (B), EpOME and DiHOME metabolites (C), and HETE metabolites and LTB4 in spinal cord tissues (D). Quantitative results were obtained by reference of experimental peak area ratios to standard curves and data were normalized to tissue weight. Data for each individual lipid were analyzed by ANOVA with Tukey's post hoc test. Error bars are SEM. **p < 0.01, ***p < 0.001 compared with untreated; #p < 0.05, ##p < 0.01 compared with NGF.