Figure 9.

Remyelination remains impaired 21 d after transfer of Th17 cells. A, Electron microscopy of corpus callosum under indicated conditions. Left, original magnification ×10,000. Scale bar, 2 μm. Right, original magnification ×50,000. Scale bar, 0.5 μm. B, The percentage of myelinated axons was determined by counting myelinated axons and total axons in 10 images ×10,000 from 4 to 6 animals per experimental group. C, Small-caliber axons were counted at ×50,000. Transfer of Th17 cells increases the abundance of small-caliber axons following cuprizone treatment. D, Total axon counts were not significantly changed across experimental groups. Data are mean ± SEM. *p < 0.05 (two-tailed Student's t test). **p < 0.005 (two-tailed Student's t test). Data are representative of two independent experiments.