Figure 1.

Schwann cell-specific deletion of Eed results in reduction of H3K27me3. A, RNA was purified from Eed cKO and control sciatic nerves at P30 and analyzed for Eed and Suz12 expression relative to 18S rRNA using qRT-PCR. Expression level of each gene in wild-type nerve is set as 1. Error bars, ±SD; ***p < 0.00001; n = 4 for control; n = 5 for Eed cKO. B, Immunohistochemistry on longitudinal sections of P15 sciatic nerves was performed for SOX10 (orange) and H3K27me3 (green) and also costained with Hoechst (blue). Arrowheads indicate Eed cKO Schwann cells with normal levels of SOX10 but greatly diminished levels of H3K27me3. The inset shows that residual H3K27me3 in Eed cKO Schwann cells is localized in speckles at heterochromatic foci of these nuclei.