Figure 4.

Gene expression analysis of Eed cKO nerves reveals tissue-inappropriate and stage-inappropriate gene expression. A, C, qRT-PCR analysis was used to identify the expression level of myelin-related genes and Pten (A) and tissue-inappropriate and stage-inappropriate genes (C) at the indicated ages. Control level is 1 at each age (data not shown). Values normalized with 18S rRNA. Data: mean ± SD; *p < 0.05, **p < 0.005, ***p < 0.0005; n = 4 for control, and n = 5 for Eed cKO at P30, and n = 5 per genotype at 4 months. B, Microarray analysis identified the induction of injury-response genes, Shh downstream genes, neuronal transcription factors, and MPNST-associated genes among derepressed genes in 4 month Eed cKO nerves. Injury-response genes were identified by genes upregulated 7 d after nerve cut in published microarray data (Arthur-Farraj et al., 2012). Data: average fold-change of three mice per genotype with adjusted p value < 0.05.