Figure 6.

Identification of H3K27me3-bound genes. A, ChIP-seq analysis was performed to identify H3K27me3-bound genes in sciatic nerves of 8-week-old wild-type mice. Transcription start site is located on the left end of gene. Each gene shown has ≥1 peaks of H3K27me3 as determined by HOMER analysis of ChIP-seq data relative to input chromatin. B, ChIP-qPCR analysis was performed to show reduction of H3K27me3 in 1 month Eed cKO sciatic nerves compared with control. Data: mean ± SD; **p < 0.005; n = 3 per genotype. An actively transcribed gene, squalene epoxidase (Sqle), in Schwann cells has a low H3K27me3 occupancy. C, List of genes that are derepressed in Eed cKO nerves (>1.5-fold) and occupied by H3K27me3 in wild-type sciatic nerves (8 wk.-SN). Black, gray fillings, and asterisks indicate H3K27me3 occupancy, neuronal differentiation genes, and injury-response genes, respectively. H3K27me3-bound genes in human neural crest cells (NCC; Rada-Iglesias et al., 2012) were annotated based on the enrichment of peaks (peak score, >20) within the genes or a 3 kb window upstream of the transcription start site .