Figure 7.

siRNA-mediated Igfbp2 reduction in Schwann cells reduces Akt phosphorylation. A, Lysates from control and Igfbp2 siRNA-treated RT4 and RSCs were subjected to Western blot. Overnight serum-starved RSCs were stimulated with neuregulin for 20 min before protein isolation. B, Quantification of Western blot. p-Akt/Akt; RT4 and RSCs, n = 6 and n = 3 per each condition, respectively. C, qRT-PCR analysis was used to identify the siRNA-mediated knockdown level in RT4. Values normalized with 18S rRNA. n = 3 per each condition. Expression of an adjacent gene, Igfbp5, was not affected by the siRNA treatment. Data: mean ± SD; *p < 0.05, **p < 0.005, ***p < 0.00005.