Skip to main content
. 2015 Jun 1;10(6):e0128552. doi: 10.1371/journal.pone.0128552

Fig 3. CA treatment rescues jejunal crypt survival after high-dose abdominal IR.

Fig 3

A. Hematoxylin and eosin (H&E)-stained jejunal sections harvested from vehicle- or CA-treated mice at 3.5 days after 12.5 Gy abdominal IR. The inserted squares depict whole cross sections of the jejunum. Original magnification, 5x. The arrows indicate crypts that survived following IR. Scale bar = 50 μm. B. Quantitative analysis of morphological changes in the jejunum following CA and IR. Data are presented as the mean ± SEM (*p < 0.05, n = 6). C. Immunohistochemical analysis of Ki-67, a proliferation marker, in a section of the jejunum at 3.5 days after IR. Ki-67 was evaluated using diaminobenzidine (DAB, brown stain) and hematoxylin counterstaining (blue). The arrows indicate crypts, representing intestinal stem cell proliferation.