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. 2015 Jun 1;10(6):e0128552. doi: 10.1371/journal.pone.0128552

Fig 5. CA confers protection against radiation-induced cell death by inducing HSF1 and HSP70 expression.

Fig 5

A. HSF1 and HSP70 expression in IEC6 cells and HUVECs was detected by Western blotting after treatment with 0–10 μM of CA at the time points indicated. B. Apoptotic cell death was measured by Annexin V/PI assay and flow cytometry at 48 h after 15 Gy IR. C. IEC6 cells and HUVECs were transfected with si-con, si-HSF1 or siHSP70 and treated with 5 μM of CA and 15 Gy of IR. Western blotting was conducted to detect cleaved caspase-3 and cleaved PARP at 48 h after 15 Gy IR in IEC6 cells and HUVECs. Actin was used as a loading control. Three different experiments were performed.