Skip to main content
. 2015 Jun 2;10(6):e0128278. doi: 10.1371/journal.pone.0128278

Fig 1. Ox-LDL induces the expression and release of ICAM-1 and MCP-1/CCL2 and up-regulates the 5-LO pathway.

Fig 1

(A) RPAECs were exposed to different concentration of ox-LDL (10–100 μg/mL) for 24 h. LTB4 protein levels in cell culture supernatant was quantified by ELISA. (B, D) 5-LO and BLT1 mRNA and protein levels were respectively measured by qRT-PCR and western blot. (C, E) ICAM-1, MCP-1/CCL2 mRNA and protein levels in cell culture supernatant were quantified by qRT-PCR and ELISA. The data shown are means ± S.E.M. of results from at least four independent experiments. *P<0.05, **P<0.01, ***P<0.001 compared with the control group.