Table 1. Sequence of forward and reverse primers for real-time PCR.
| Gene1 | Gene accession no. | Sequence (5’-3’) | |
|---|---|---|---|
| ABCA1 | NM_001024693 | Forward | GGACATGTGCAACTACGTGG |
| Reverse | TGATGGACCACCCATACAGC | ||
| ABCG1 | XM_587930 | Forward | GACTCGGTCCTCACGCAC |
| Reverse | CGGAGAAACACGCTCATCTC | ||
| GAPDH | NM 001034034 | Forward | TACATGGTCTACATGTTCCAGTATG |
| Reverse | CAGTCTTCTGGGTGGCAGTGATG | ||
| HMGCR | BC153262 | Forward | ACCCATGAGCGAGGTGTATC |
| Reverse | TAGTGCTGGCCACAAGACAG | ||
| HMGCS1 | BC102850 | Forward | TGTACGGCTCCCTGGCTTCTG |
| Reverse | CATGTTCCTTCGAAGAGGGAATC | ||
| LXRα | NM_001014861.1 | Forward | CTGCGATTGAGTGATGCTC |
| Reverse | CGGTCTGCAGAGAAGATGC | ||
| MTTP | NM_001101834.1 | Forward | TCATCCAATGTGGATGTCGC |
| Reverse | GAGATTTTCTATGGCTGCTG | ||
| PPARα | NM_001034036.1 | Forward | AGGGCTGCAAGGGTTTCTTTAG |
| Reverse | TGACGAAAGGCGGGTTGTTGTTG | ||
| PPARγ | NM_181024.2 | Forward | AACTCCCTCATGGCCATTGAATG |
| Reverse | AGGTCAGCAGACTCTGGGTTC | ||
| SREBF1 | NM_001113302.1 | Forward | CCAGCTGACAGCTCCATTGA |
| Reverse | TGCGCGCCACAAGGA | ||
| SREBF2 | XM_583656.3 | Forward | GACTGATGCCAAGATGCACA |
| Reverse | CCCTTCAGGAGTTTGCTCTT | ||
| UBQ | Z18245 | Forward | AGATCCAGGATAAGGAAGGCAT |
| Reverse | GCTCCACTTCCAGGGTGAT |
1 ABCA1: ATP-binding cassette transporter A1; ABCG1: ATP-binding cassette transporter G1; GAPDH: glycerinaldehyd-3-phosphat-Dehydrogenase; HMGCR: 3-hydroxy-3-methylglutaryl-coenzyme A reductase; HMGCS1: 3-hydroxy-3-methylglutaryl-coenzyme A synthase 1; LXRα: liver X receptor α; MTTP: microsomal triglyceride transfer protein; PPARα: peroxisome proliferator activated receptor α; PPARγ: peroxisome proliferator activated receptor γ; SREBF-1: sterol regulatory element-binding factor 1; SBREF-2: sterol regulatory element-binding factor 2; UBQ: ubiquitin.