Skip to main content
. 2015 Jun 2;10(6):e0127283. doi: 10.1371/journal.pone.0127283

Fig 5. Expression of Osteopontin RNA and Protein in BAL After Ozone Exposure.

Fig 5

A. Osteopontin gene (SPP1) RNA expression increased with increasing ozone exposure (q-value of 9.1x10-7 in linear regression analysis). B. Immunoblot of BAL samples from four representative subjects after exposure to 0, 100, and 200 ppb ozone probed with anti-OPN antibody. C. Osteopontin total protein (monomeric and polymeric forms) signal (from immunoblot densitometry analysis) increased with increasing ozone exposure (linear regression p-value = 0.03). D & E. The ratio of polymeric to monomeric forms of osteopontin decreased with increasing ozone exposure in those subjects with asthma (linear regression p-value = 0.01). Bars represent Mean ± SEM.