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. 2015 Jun 2;10(6):e0127283. doi: 10.1371/journal.pone.0127283

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Fig 6 — 16HBE14o- cells were grown to confluence on trans-well membrane plates and used in a scratch assay as a model of wound closure. A. Open wound area was measured 14 hours after scratch and treatment with saline (PBS), monomeric osteopontin (mOPN), transglutaminase 2 (TG2), polymeric osteopontin (pOPN), with and without anti-osteopontin antibody (anti-OPN) or anti-α9 integrin antibody (anti-α9). The scale bar is 50 μm. B. Average width of open wound area was calculated by adjusting wound area for length of scratch. The graph shows mean (middle horizontal bar) and standard deviation (whiskers).