Table 3. Differentially Expressed Genes with Increasing Levels of Ozone Exposure.
| Regression Analysis | PADE Pair-wise Analyses | |||||
|---|---|---|---|---|---|---|
| Gene Descriptor | 100 ppb O3 (fold change) | 200 ppb O3 (fold change) | Regression q-value | 0 vs. 100 ppb O3 (fold change) | 100 vs. 200 ppb O3 (fold change) | 0 vs. 200 ppb O3 (fold change) |
| PADE and Regression Significant | ||||||
| SPP1 | 2.12 | 5.96 | <0.001 | 2.14 | 2.68 | 5.73 |
| CCL2 | 1.21 | 2.82 | 0.03 | 1.26 | 2.31 | 2.9 |
| IL8RA | 1.24 | 2.48 | 0.01 | 1.34 | 2.21 | 2.96 |
| S100A12 | 1.20 | 2.41 | <0.001 | 1.25 | 2.22 | 2.78 |
| PLXNC1 | 1.17 | 2.24 | <0.001 | 1.19 | 1.98 | 2.36 |
| CD1E | 1.31 | 2.17 | <0.001 | 1.31 | 1.7 | 2.23 |
| MERTK | 1.13 | 2.10 | <0.001 | 1.17 | 1.93 | 2.26 |
| CD1C | 1.24 | 2.08 | <0.001 | 1.24 | 1.7 | 2.11 |
| IL1R2 | 1.22 | 2.07 | <0.001 | 1.31 | 1.77 | 2.33 |
| RASSF2 | 1.11 | 1.94 | 0.02 | 1.09 | 1.85 | 2.01 |
| KCNJ15 | 1.17 | 1.89 | 0.01 | 1.28 | 1.65 | 2.12 |
| GPR84 | 1.10 | 1.63 | 0.01 | 1.16 | 1.56 | 1.81 |
| ACPP | 1.09 | 1.63 | <0.001 | 1.06 | 1.61 | 1.7 |
| ST8SIA4 | 1.00 | 1.53 | 0.03 | -1.01 | 1.55 | 1.54 |
| MEF2C | -1.01 | 1.52 | 0.02 | -1.03 | 1.59 | 1.54 |
| Regression Significant Only | ||||||
| PLA2G7 | 1.43 | 2.65 | <0.001 | 1.48 | 1.75 | 2.59 |
| LAMP3 | 1.28 | 1.82 | <0.001 | 1.31 | 1.4 | 1.84 |
| CCL22 | 1.24 | 1.81 | <0.001 | 1.24 | 1.52 | 1.89 |
| PI3 | 1.21 | 1.70 | 0.04 | 1.26 | 1.52 | 1.91 |
| CX3CR1 | 1.18 | 1.69 | <0.001 | 1.18 | 1.53 | 1.8 |
| MMP9 | 1.31 | 1.68 | 0.01 | 1.36 | 1.33 | 1.82 |
| PRKCB | 1.09 | 1.60 | 0.01 | 1.1 | 1.5 | 1.66 |
| CHST15 | 1.14 | 1.54 | 0.04 | 1.17 | 1.43 | 1.67 |
| ETV5 | 1.22 | 1.51 | 0.02 | 1.25 | 1.24 | 1.55 |
| MGAM | 1.16 | 1.45 | 0.02 | 1.18 | 1.26 | 1.49 |
| MMP8 | 1.20 | 1.45 | 0.03 | 1.25 | 1.2 | 1.5 |
| HSD11B1 | 1.32 | 1.42 | <0.001 | 1.35 | 1.08 | 1.46 |
| ANKH | 1.03 | 1.29 | 0.01 | 1.01 | 1.3 | 1.32 |
| SIGLEC10 | 1.06 | 1.29 | 0.01 | 1.06 | 1.25 | 1.33 |
| SEMA4A | 1.10 | 1.24 | 0.02 | 1.09 | 1.18 | 1.29 |
| PADE Significant Only | ||||||
| STEAP4 | 1.18 | 2.24 | 1.00 | 1.40 | 1.89 | 2.65 |
| GPR183 | 1.19 | 1.95 | 0.07 | 1.18 | 1.69 | 2.00 |
| ANKRD22 | 1.14 | 1.82 | 0.17 | 1.12 | 1.75 | 1.96 |
| CCR2 | 1.04 | 1.73 | 0.34 | -1.01 | 1.79 | 1.78 |
| SERPINB9 | 1.08 | 1.72 | 0.11 | 1.05 | 1.67 | 1.75 |
| C4orf18 | 1.06 | 1.64 | 0.14 | 1.01 | 1.7 | 1.73 |
| CD62L | -1.01 | 1.59 | 1.00 | 1.00 | 1.73 | 1.72 |
| SELL | 1.00 | 1.59 | 1.00 | 1.01 | 1.7 | 1.72 |
| CORO1A | 1.00 | 1.54 | 1.00 | -1.05 | 1.69 | 1.61 |
| CLEC5A | 1.02 | 1.51 | 1.00 | -1.02 | 1.6 | 1.56 |
| SLC25A37 | -1.01 | 1.49 | 0.79 | 1.03 | 1.6 | 1.65 |
| SULF2 | 1.13 | 1.48 | 0.06 | 1.11 | 1.42 | 1.57 |
| FAM65B | -1.02 | 1.39 | 1.00 | -1.01 | 1.53 | 1.51 |
| LILRA1 | 1.02 | 1.38 | 0.07 | -1.01 | 1.47 | 1.46 |
| IFITM2 | -1.04 | 1.21 | 1.00 | -1.04 | 1.37 | 1.33 |
The significant differentially expressed genes (DEGs) from both regression and PADE pair-wise analyses are listed with fold changes and q-values from regression analysis, as well as PADE delta values and fold changes by PADE analysis. A fold change of 1.00 would reflect no change. The DEGs are separated into three sections: genes found to be differentially expressed by both linear regression and PADE analyses; genes found to be differentially expressed only by linear regression analysis (q-value), and genes found to be differentially expressed only by PADE delta analysis. For PADE pairwise analyses, only DEGs that were consistently regulated across all three pairwise comparisons with PADE delta values above the significance thresholds in each of the two-group comparisons are shown. P-value for regression analysis fold changes <0.001; PADE Delta significance threshold for FA vs 200 ppb two-group comparison = 0.7; PADE Delta significance threshold for 100 ppb vs 200 ppb two-group comparison = 0.5; PADE Delta significance threshold for FA vs 100 ppb two-group comparison = 0.2. Genes highlighted in bold represent the DEGs whose expressions were also up-regulated in the separate preliminary study of 3 subjects (see Methods and S2 Table).