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. 2015 Jun 3;6:119. doi: 10.3389/fphar.2015.00119

FIGURE 5.

FIGURE 5

BVR enhances ERK1/2-dependent transcriptional activation. (A) NF-κB-dependent promoter activity is stimulated by BVR. Cells were cotransfected with a luciferase reporter plasmid regulated by NF-κB, a β-Gal reporter (to serve as a control for transfection efficiency), and either pcDNA-hBVR or empty vector. 24 h later, NF-κB-dependent promoter activity was measured by luciferase assay and normalized to β-galactosidase. Modified from (Gibbs and Maines, 2007). (B) BVR-siRNA suppresses activation of NF-κB by PKCδ. Cells were cotransfected with NF-κB and β-gal reporters, pcDNA-PKCδ and siRNA for BVR or control scRNA. Promoter function was analyzed as in (A), *p < 0.001. Modified from (Gibbs et al., 2012a). (C) TNF-α-mediated induction of the iNOS gene is potentiated by BVR. Cells were cotransfected with pcDNA3-hBVR, a luciferase reporter plasmid wherein luciferase is regulated by the iNOS promoter, and β-gal reporter. 12 h after DNA addition, cells were treated with TNF-α for a further 12 h. Promoter function was then analyzed as in (A). Modified from (Gibbs and Maines, 2007).