Table 2.
M-PCR validation based compared to sPCR for thirteen major BV-AAs in cervical-vaginal samples.
| Agents | Sensibility (%) | Specificity (%) | PPV (%) | NPV (%) | Accuracy (%) |
|---|---|---|---|---|---|
| Overall | 99.1 | 100.0 | 100.0 | 97.0 | 99.3 |
| Mobiluncus curtisii | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 |
| Ureaplasma urealyticum | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 |
| Mycoplasma hominis | 80.0 | 100.0 | 100.0 | 97.8 | 98.0 |
| Gardnerella vaginalis | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 |
| Megasphaera type 1 | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 |
| Bacteroides fragilis | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 |
| Atopobium vaginae | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 |
| BVAB1 | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 |
| BVAB2 | 88.8 | 100.0 | 100.0 | 97.6 | 98.0 |
| BVAB3 | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 |
| Mycoplasma genitalium | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 |
| Mobiluncus mulieris | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 |
| Sneathia sanguinegens | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 |
| Two or more agents simultaneously | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 |
M-PCR: multiplex polymerase chain reaction; sPCR: single polymerase chain reaction; BV-AAs: bacterial vaginosis-associated agents; PPV: positive predictive value; NPV: negative predictive value.