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. 2015 May 20;2015:167308. doi: 10.1155/2015/167308

Figure 5.

Figure 5

NX1β differentially affects the phosphorylation and expression of NR1. (a) Cortical neurons were grown for 8 days, subsequently treated with different concentrations of recombinant NX1β for 5 min, and further immunoblotted for the detection of NR1 phosphorylation at S896 and S897. Representative immunoblots of three to four individual experiments are shown. The results from n ≥ 4 experiments are expressed as a percentage ± SEM. P < 0.05, compared with the untreated control set at 100%. (b) Cortical neurons were grown for 8 days and treated with different concentrations of recombinant NX1β for 48 h. Total RNA was isolated, and mRNA levels were assessed using RT-PCR. The results from n = 5 experiments are expressed as a percentage ± SEM. P < 0.05, compared with the untreated control set at 100%.