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NX1β activates ERK1/2 and Akt kinases. Cortical neurons were grown for 8 days, subsequently treated with different concentrations of recombinant NX1β for 5 min, and further immunoblotted for the detection of (a) ERK1/2, (b) p38 MAPK, and (c) Akt. (d) Effect of the PKA inhibitor H89 on NX1β-induced ERK1/2 phosphorylation. The results from n ≥ 4 experiments are expressed as a percentage ± SEM. ∗ P < 0.05, compared with the untreated control set at 100%.
