Figure 6. Effect of a p51 thumb' domain mutation on the Ile methyl resonances in the p66 subunit of RT.

(A) Overlay of the ¹H-¹³C HMQC spectrum of [¹³CH₃-Ile]p66/p51 (green) and [¹³CH₃-Ile]p66/p51(L289K) (black). Most of the features of the spectrum are preserved, consistent with the formation of a stable heterodimer. The labeling pattern corresponds to the color-coding in the cartoons below the spectrum. (B) Ribbon diagram illustrating the relative position of the mutated residue (blue sphere) and perturbed resonances in the RH and connection domains of p66 (orange spheres). Color coding: p51 (blue); p66 RH domain (magenta); p66 connection domain (yellow), p66 fingers/palm and thumb domains (green).

DOI: http://dx.doi.org/10.7554/eLife.06359.027