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. 2015 May 7;7(5):2321–2333. doi: 10.3390/v7052321

Figure 2.

Figure 2

Figure 2

Alphavirus vector systems. (A) Replication-deficient recombinant virus particles: recombinant particles are generated by co-transfection of in vitro transcribed RNA from expression and helper vectors. The replication-deficient particles are capable of transduction of host cells resulting in high-level transient expression of recombinant protein. (B) Replication-proficient recombinant virus particles: transfection of RNA including the full-length alphavirus genome and the gene of interest generates replication-proficient particles capable of recombinant protein expression and simultaneous virus production after transduction of host cells. (C) Layered DNA/RNA vectors: transfection of vector DNA provides transient expression of recombinant protein.

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