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. 2015 May 13;7(5):2378–2403. doi: 10.3390/v7052378

Figure 4.

Figure 4

Influence of proteosome inhibitors on virus release. (A) PT cells infected with WT BTV at 0.1 MOI were incubated for 10 or 14 h and treated with MG132 or lactacystin for 2 h. Total and released virus titres were assessed by plaque assay and the significant difference in the virus titres were designated by asterisk (*). The plaque assays were done in triplicate and bars represent standard error; (B) Relative virus titres of (A); (C–F) Expression of free ubiquitin (C,D) and VP5, NS2, actin (E,F) in treated and infected PT cells. The cells were treated similar to A,B. Lysates were analyzed by SDS-PAGE and Western blotting. Molecular masses and proteins are indicated on left and right, respectively. Presence or absence of diluent DMSO and inhibitors has been indicated by (+) or (−), respectively. Arrows represent presence of ubiquitin in untreated cells. NI signified uninfected cells.