Figure 5.

Dengue viral titer determined using qRT-PCR in serum or tissues collected from UV-12 treated AG129 mice. Groups of AG129 mice received the first treatment dose of 100 mg/kg of UV-12 or vehicle control 1 h before infection with DENV2 at a dose of ~1LD₉₀. Treatment continued three times daily at 8 h intervals until tissue harvest at 72 or 92 h post infection. Viral titers in the serum or liver, spleen, kidney and small intestines were determined by qRT-PCR for each sample from individual animals and the mean with standard deviation are also shown for each group. Data are shown as log DENV genome equivalents per mL of serum or as log DENV genome equivalents per relative 18S (× 10⁴) for the tissues analyzed. Statistical differences (p < 0.05) are indicated with the actual values over the relevant data points.