Table 1.
Comparison of hemangioblasts and hemogenic endothelial cells in mouse
| location | features | hemangioblasts extraembryo | hemogenic endothelial cell | references | |
|---|---|---|---|---|---|
| extraembryo intraembryo | |||||
| In vivo | time | E7.0 - E7.75 mid-streak stage of gastrulation | E8.25 lumen wall of YK capillaries | E10.5 - E11.5 ventral wall of the dorsal aortic endothelium | [Adamo et al., 2009; Huber et al., 2004; Iacovino et al., 2011; Kim et al., 2013; Liu et al., 2013; Marcelo et al., 2013; Tsunoda et al., 2010; Yue et al., 2012] |
| phenotype | brachyury+ Flk-1+ | Flk1+c-Kit+ CD45–Aldh+ SP cells | Flk-1+c-Kit+CD31+CD45–CD34+AA4.1+/VEC+Aldh+ SP cells | ||
| differentiation potential | primitive hematopoiesis and endothelium smooth muscle | definitive hematopoiesis and endothelium | definitive hematopoiesis endothelium | ||
| transcript factors | Flk1, Hhex,Mixl1, Smad1, Scl,Runx1, Gata1,lmo2, ZFAT | Scl/TAL1, Hhex, Runx1,Gata2, Gata3,c-Myb, Gfi1,Gfi1b | Sox17 ,HoxA3, Scl, Runx1,Gata2, Gfi1, Gfi1b, Fli1 | ||
| signaling pathways | ER71, VEGF/Flk1, FGF, BMP, Notch, Wnt, Hedgehog (Hh), Spry1 | RA/c-kit/Notch1/p27 | RA, Notch1, Wnt, BMPs, Hedgehog (Hh), NO signaling, VEGF, IL-1, IL-3 | ||
| In vitro (ESC) | time phenotype differentiation potential | 3.25-4 days of EB Flk-1+ BL-CFU smooth muscle cardiomyocyte mural cells | after 36-48 hrs cultured hemangioblasts Tie2+c-Kit+ CD41- definitive hematopoiesis endothelium | [Adamo et al., 2009; Choi et al., 1998; Faloon et al., 2000; Goldie et al., 2008; Kim et al., 2013; Lancrin et al., 2009; Liu et al., 2013; Marcelo et al., 2013; Pearson et al., 2008; Pimanda et al., 2007; Yue et al., 2012] | |
| transcript factors | FGF2, activin A, Flk1, Scl, Runx1, Gata1,Gata2,Lmo2, Hhex,Mixl1, Smad1, Lycat | Scl/TAL1,HoxA3,Runx1, GATA2, LMO2, Gfi1 and Gfi1b | |||
| signaling pathways | ER71,VEGF/Flk1,FGFR1, BMP, Notch, Wnt, Hedgehog (Hh), phosphatidylinositol 3-kinase | Hedgehog(Hh), Notch-1, NO signaling, F2r-RhoA/ROCK | |||