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. Author manuscript; available in PMC: 2016 Jan 15.
Published in final edited form as: Cell. 2015 Jan 15;160(0):88–104. doi: 10.1016/j.cell.2014.12.022

Figure 5. Denervation of DKO inguinal WAT and increases body weight.

Figure 5

a–d) IngWAT in 10 week-old DKO mice was unilaterally denervated (6-ODHA) and BAT and ingWAT extracted 2 weeks later for analysis by a) gross morphology, b) histology, immunohistochemistry and c–d) real time PCR; vehicle administered contralateral inguinal fat pads were used as controls. e) Contralateral sham versus denervated (6-ODHA) ingWAT from DKO versus Ptpn1fl/flPtpn2fl/fl mice was analysed for Ucp-1 expression. f–h) 8 week-old Ptpn1fl/flPtpn2fl/fl and DKO mice were sham-operated or bilaterally denervated and (f) body weights, g) BAT and WAT weights assessed and h) ingWAT and BAT Ucp-1 expression measured. i) Energy expenditure and oxygen consumption in 24 week-old Ptpn1fl/flPtpn2fl/fl and DKO mice 5 weeks after sham or bilateral ingWAT denervations. Data are means ± SEM and are representative of 3 independent experiments; significance determined using e–f) two-way, or g–i) one-way ANOVA. f) * corresponds to DKO v/s Ptpn1fl/flPtpn2fl/fl; # denervated DKO v/s sham-operated DKO.