TABLE 2 .
In vitro antifungal susceptibility profiles of A. fumigatus isolates and corresponding SNPs in cyp51A with nonsynonymous substitutions
| Culture reference |
MIC (mg/liter) ofa: |
cyp51A amino acid substitution at position: |
Resistance marker |
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ITC | VOR | POS | ISA | CAS | MFG | AFG | AMB | F46 | L98 | M172 | N248 | D255 | S297 | E427 | F495 | ||
| 12-7505446 | >16 | 1 | 0.5 | ND | 0.125 | <0.015 | <0.015 | 0.25 | Y | H | V | T | E | S | K | F | TR34/L98H |
| 12-7505220 | >16 | 1 | 0.5 | ND | 0.125 | <0.015 | <0.015 | 0.5 | Y | H | V | T | E | S | K | F | TR34/L98H |
| 09-7500806 | 1 | 0.25 | 0.06 | ND | 0.06 | <0.015 | <0.015 | 0.5 | Y | L | V | T | E | S | K | F | None |
| 12-7504652 | 1 | 0.5 | 0.25 | ND | 0.125 | <0.015 | <0.015 | 0.25 | Y | L | V | T | E | S | K | F | None |
| 12-7504462 | 0.5 | 0.125 | 0.06 | ND | 0.125 | <0.015 | <0.015 | 0.5 | Y | L | V | T | E | S | K | F | None |
| 12-7505054 | 0.5 | 0.5 | 0.06 | ND | 0.5 | 0.063 | 0.06 | 0.5 | Y | L | V | T | E | S | K | F | None |
| 08-12-12-13 | >16 | 1 | 1 | 16 | 0.5 | 0.063 | 0.031 | 0.25 | Y | H | V | T | E | T | K | I | TR34/L98H |
| 08-36-03-25 | >16 | 1 | 0.5 | 8 | 0.5 | 0.008 | 0.008 | 0.5 | Y | H | V | T | E | T | K | I | TR34/L98H |
| 08-31-08-91 | >16 | 4 | 1 | 8 | 0.25 | 0.031 | 0.016 | 0.5 | Y | H | V | T | E | S | K | F | TR34/L98H |
| 08-19-02-61 | >16 | 2 | 0.25 | 4 | 0.5 | 0.031 | 0.008 | 0.25 | Y | H | V | T | E | S | K | F | TR34/L98H |
| 08-19-02-30 | 0.25 | 0.5 | 0.06 | 0.5 | 0.5 | 0.016 | 0.016 | 0.5 | Y | L | V | T | E | S | K | F | None |
| 10-01-02-27 | >16 | 4 | 0.5 | 4 | 0.5 | 0.016 | 0.016 | 0.5 | Y | H | V | T | E | S | K | F | TR34/L98H |
| 08-19-02-46 | >16 | 4 | 0.5 | 4 | 0.5 | 0.016 | 0.016 | 0.5 | Y | H | V | T | E | S | K | F | TR34/L98H |
| 08-19-02-10 | >16 | 2 | 0.5 | 4 | 0.5 | 0.031 | 0.016 | 0.25 | Y | H | V | T | E | S | K | F | TR34/L98H |
| Afu 942/09 | >16 | 2 | 2 | 8 | 0.125 | <0.015 | <0.015 | 0.25 | Y | H | V | T | E | S | K | F | TR34/L98H |
| Afu 1042/09 | >16 | 2 | 2 | 8 | 0.06 | <0.015 | <0.015 | 0.25 | Y | H | V | T | E | S | K | F | TR34/L98H |
| Afu 343/P/11 | >16 | 8 | >8 | >8 | 0.125 | <0.015 | <0.015 | 0.125 | Y | H | V | T | E | S | K | F | TR34/L98H |
| Afu 591/12 | >16 | 8 | >8 | 2 | 0.25 | ≤0.015 | 0.06 | 0.5 | Y | H | V | T | E | S | K | F | TR34/L98H |
| Afu 124/E11 | >16 | 8 | 1 | 8 | 0.06 | <0.015 | <0.015 | 0.125 | Y | H | V | T | E | S | K | F | TR34/L98H |
| Afu 166/E11 | 16 | 16 | 2 | 8 | ND | ND | ND | ND | Y | H | V | T | E | S | K | F | TR34/L98H |
| Afu 257/E11 | >16 | 8 | 1 | >8 | 0.125 | <0.015 | <0.015 | 0.125 | Y | H | V | T | E | S | K | F | TR34/L98H |
| Afu 218/E11 | >16 | 8 | 1 | >8 | 0.125 | <0.015 | <0.015 | 0.125 | Y | H | V | T | E | S | K | F | TR34/L98H |
| AF65 | 0.5 | 0.125 | 0.06 | ND | 0.125 | <0.015 | <0.015 | 0.5 | Y | L | V | T | E | S | K | F | None |
| AF293 | 0.5 | 0.25 | 0.06 | ND | 0.125 | <0.015 | <0.015 | 0.25 | F | L | M | N | D | S | E | F | None |
a
ITC, itraconazole; VOR, voriconazole; POS, posaconazole; ISA, isavuconazole; CAS, caspofungin; MFG, micafungin; AFG, anidulafungin; AMB, amphotericin B; ND, not done.