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. 2015 Jun 2;6(3):e00531-15. doi: 10.1128/mBio.00531-15

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Copyright © 2015 Lev et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 1 — Kcs1 is an IP₆ kinase in C. neoformans. (A) Phylogenetic analysis of Kcs1 homologs in fungi. Full-length C. neoformans Kcs1 protein (indicated by the arrow) was used to generate the phylogenetic tree. Cg, Candida glabrata XP_445605; Sc, Saccharomyces cerevisiae Q12494; Af, Aspergillus fumigatus XP_753330; An, Aspergillus nidulans XP_664484; Ci, Coccidioides immitis EAS27460.2; Um, Ustilago maydis XP_757030.1; Cc, Coprinopsis cinerea XP_001836888; Pc, Phanerochaete carnosa EKM59468; Ta, Trichosporon asahii EKD02283; Cn, Cryptococcus neoformans AFR93794. Numbers represent bootstrap values. (B) Analysis of the inositol polyphosphate profile of the WT, Δkcs1, and Δarg1 strains. The inset represents IP profiles of the reconstituted Δkcs1 strains: the Δkcs1::KCS1 strain (expressing native Kcs1) and the Δkcs1::KCS1^AAA strain (expressing kinase-inactive Kcs1). Intracellular IPs were labeled with [³H]myo-inositol, extracted, and analyzed by anion-exchange HPLC. The elution time for different IP species was determined using standards. The position of each IP species is indicated.