FIG 4 .
Kcs1 deficiency is associated with a mucoid (glossy) colony appearance (A) and enlarged capsules (B and C). (A) Fungal strains were grown on Sabouraud (SAB) agar plates at ambient temperature for 3 days. (B) The capsules of the SAB agar-grown cells were visualized by negative staining with India ink. (C) Capsule width was measured in at least 30 cells. The increases in capsule sizes observed for the Δkcs1 and Δkcs1::KCS1AAA strains, relative to the WT and Δkcs1::KCS1 strains, respectively, are statistically significant (*, P < 0.05), as determined using a Tukey-Kramer multiple-comparison test. Error bars indicate standard errors.