Fig. 1. Inhibition of airway responsiveness, lung inflammation, and inflammatory cell infiltration in mice treated by adoptive transfer of HspX-DCs. (A) Airway responsiveness was measured 24 h after the final challenge in saline-inhaled mice administered PBS (Control), OVA-sensitized mice administered saline (OVA), OVA-sensitized mice transferred with OVA-non pulsed DCs (DCs), OVA-sensitized mice transferred with OVA-pulsed DCs (OVA-DCs), and OVA-sensitized mice transferred with OVA-pulsed HspX-stimulated DCs (HspX-DCs). Enhanced pause (Penh) values were determined. Data are means ± standard error of the mean (SEM) from three independent experiments. (B) Cells from bronchoalveolar lavage (BAL) fluid were collected and total differential inflammatory cell counts were conducted. (C) Lungs were removed 48 h after the final airway challenge. Sections were stained with haematoxylin and eosin (H & E), and periodic acid-Schiff (PAS) (400×). Representative results were of three mice per group from three independent experiments. Results are expressed as mean ± SEM.