Fig. 2. Adoptive transfer of HspX-DCs modulates cytokines. Mice were treated as described in the Materials and Methods section. BAL fluid from each group of mice was obtained 48 h after the final airway challenge. TNF-α (A), IFN-γ (B), IL-12p70 (C), IL-13 (D), IL-17A (E), and IL-6 (F) levels in the BAL fluid were measured by enzymelinked immunosorbent assay (ELISA) according to the manufacturer’s instructions. Results are means ± SEM from three experiments. Statistical significance was assessed by Student’s paired t test, which was used to determine differences between two groups. The Turkey-Kramer test was used for comparisons between multiple groups. *P ＜ 0.05, **P ＜ 0.01, and ***P ＜ 0.001.