Fig. 3. Effect of adoptive transfer of HspX-DCs on the expression level of GATA-3 and T-bet, and immunoglobulin production. (A) Blood was collected by cardiac puncture, and serum IgE and IgG2a levels were analyzed using ELISA (n = 5). Data are means ± SEM from three experiments. **P ＜ 0.01 and ***P ＜ 0.001. (B) Samples were obtained 2 days after the last OVA challenge from mice that received PBS (control), non-pulsed DCs with OVA (DCs), OVA pulsed DCs (OVA-DCs), or OVA pulsed and HspX -stimulated DCs (HspX-DCs), and were subjected to western blot analysis with α-GATA-3 or α-T-bet antibody.