Fig. 4. Effect of HspX-DCs adoptive transfer on Treg cells and production of TGF-β in allergic asthma mouse model. (A) Mediastinal lymph nodes were obtained 2 days after the last OVA challenge, and treated with collagenase IV. Cells were stained with CD4, CD25 and Foxp3 antibodies. Representative results of three independent experiments. (B) BAL fluid from each group of mice was obtained 48 h after the final airway challenge. TGF-β levels in the BAL fluid were measured by ELISA according to the manufacturer’s instructions. Results are expressed as mean ± SEM. *P ＜ 0.05, **P ＜ 0.01 and ***P ＜ 0.001.
