Fig. 3. Effects of Mcl-1 knockdown on proliferation of MeT-5A and MSTO-211H cells. Cells were transfected with 10 nM Mcl-1-targeting siRNA (siMcl-1) or Stealth RNAi control (siC) for 24 h. (A) Cells were incubated with resveratrol (15 μM) and clofarabine (40 nM) for 1, 3, and 5 days, after which they were processed for MTT assay. (B) Cells were incubated with resveratrol (15 μM) and clofarabine (40 nM) for 24 h. Whole cell lysates were obtained and subjected to Western blot analysis using antibodies against Mcl-1, PARP, procaspase-3, cleaved caspase-3, and β-actin. Representative results from one of three independent experiments were shown. Error bars represent the mean ± SEM for three independent experiments. Cl/R, clofarabine plus resveratrol. *P ＜ 0.05 compared to respective controls.
